【摘要】 目的为了探讨邻苯二甲酸二乙基己酯(DEHP)导致生物机体内不同器官DNA-蛋白质的交联(DPC)作用。方法以采用KCl-SDS沉淀法来检测DEHP体外染毒后昆明纯系小鼠体内肝、脑、睾丸、肺等4个器官的细胞中DNA-蛋白质交联含量。结果当DEHP的浓度为5μM时,小鼠肝细胞的DPC系数与对照组相比没有发生显著的变化;当DEHP的浓度分别升高到10、20、40和80μM时,DPC系数显著上升,且DPC含量与对照组相比有极显著差异;在脑细胞中,当DEHP的浓度分别为5、10μM时,能显著诱导DPC的生成(P<0·05),但是,随着DEHP的浓度升高,DPC的含量与对照组相比没有显著性差异;在睾丸的细胞中,当DEHP的浓度为5μM时,小鼠睾丸的细胞DPC系数与对照组相比有显著的升高(P<0·05);当DEHP的浓度升高到10、20、40、80μM时,DPC系数显著上升,且DPC含量与对照组相比有极显著差异(P<0·01);在肺细胞中,DEHP的浓度为5μM时,就能显著诱导DPC的生成(P<0·05)。结论DNA-蛋白质交联是一种严重的DNA损伤,DEHP可诱导DNA-蛋白质交联的产生。更多还原

【Abstract】 Objective In order to study the effects of di(2 ethylhexyl)phthalate (DEHP) induced DNA-protein crosslink (DPC) in mice organs. Methods KCl-SDS method was applied to detect DPC contents in livers, brains, testis and lungs of Kunming mice exposed to DEHP. Results The results showed that at the presence of DEHP as 5 μM, DPC coefficient in mice liver cells were comparative with control group.However, DPC coefficient was significantly increased when DEHP concentrations were up to 10, 20, 40 and 80 μM (compared with control group, P<0.01). In brain cells, DEHP induced DPC formation was significantly low (as low as 5 μm and 10 μM). Contrast to the results obtained in liver cells, DPC contents were not elevated significantly with the increase of DEHP levels. In testis, when DEHP concentration was 5 μM, DPC coefficient was significantly elevated (compared with control group, P<0.05); when up to 10 μM, 20 μM, 40 μM and 80 μM, DPC coefficient increased (compared with control group, P<0.01).Conclusion In lung cells, DEHP could induce DPC formation significantly as low as 5 μM. These results indicated that DEHP could induce DPC formation.更多还原