【摘要】 目的研究卵巢巧克力囊肿异位内膜腺上皮、间质细胞及其在位内膜P450芳香化酶蛋白和mRNA表达, 探讨其在子宫内膜异位症发病中的作用及其临床意义。方法采用逆转录聚合酶联反应(RT-PCR)和免疫组化技术检测45例巧囊壁、32在位内膜和35例正常内膜中P450芳香化酶(P450A)mRNA及蛋白的表达。结果巧囊壁异位内膜P450A mRNA表达量高于在位内膜(P<0．01);35例正常内膜中仅有2例表达;免疫组化检测巧囊壁P450A蛋白其表达特征与mRNA一致。结论子宫内膜异位症P450A的表达在其发病中起到重要作用。更多还原

【Abstract】 Objective To study the expression of P450arom (P450A) protein and mRNA in human ovarian endometriosis, eutopic and normal endometrium. Methods PT - PCR was used to assess P450arom messenger RNA(mRNA) expression of 45 ovarian endometriosis and 35 normal eutopic endometrium; immunohistochemical assay was performed to locate and examin the protein expression of P450arom in the above cases. Results P450A mRNA level was higher in the ectopic endometrium than that in eutopic endometrium( P < 0.01) ; P450A mRNA was present in only 2 of 35 normal uterine endometrium samples; The expression characteristic of P450A protein is the same as one of P450A mRNA in ectopic endometrium. Conclusion The abnormal expression of P450arom probably play an important role in the development and growth of endometriosis.更多还原