【摘要】 目的研究副肿瘤综合征(PNS)自身抗体(抗Hu抗体)对培养神经细胞的直接影响。方法抗Hu抗体阳性PNS患者血清IgG、对照组血清IgG添加到由胎鼠脑分离出的培养神经细胞,观察有无细胞的凋亡。对照组血清IgG为：(1)AB型健康人标准血清；(2)无神经损害症状的抗Hu抗体阴性痛患者血清；(3)重组Hu蛋白吸附后的抗Hu抗体阳性患者血清。结果添加抗Hu抗体阳性PNS患者血清IgG的培养神经细胞互相融合,由粗而长的突起相互连接,而对照组未看到上述变化。各组均未发现凋亡的神经细胞。结论抗Hu抗体未能引起神经细胞的凋亡或死亡,推测是诱导了接触因子的表达促进了细胞的分化。更多还原

【Abstract】 Objective To examine the direct effects of the autoantibody(anti-Hu antibody)of paraneoplastic neurological syndrome on primary-cultured neurons.Methods The serum IgG from PNS patients with anti-Hu antibody and control groups cultured in mouse-brain-derived neurons to observe apoptosis respectively.The serum IgG of control groups came from(1)AB blood type of healthybodies;(2) cancer patients without neurological symptoms anti-Hu antibody negative;(3)antibodyabsorbed sera from Hu-IgG patients with Hu recombinant protein.Results Neurons incubated with Hu-IgG started to fuse and extended long and thick dendrites,which were not found in the control groups.No apoptotic neurons were found in each group.Conclusion Anti-Hu antibody did not kill neurons,but induced the expression of cell adhesion molecules and accelerated neuronal differentiation.Suggesting that anti-Hu antibody was not through the binding of the antibody to specific antigen,but in IgG fraction some other factors were contained.更多还原