【摘要】 目的以体外敏感性试验结果指导化疗病人与常规化疗病人进行对照研究,评价肿瘤细胞体外敏感与体内化疗作用的相关性。方法选择62例行肿瘤全切除术或次全切除术的脑胶质细胞瘤患者作为试验组,对其手术切除的肿瘤标本先进行体外细胞培养,然后应用MTT比色法进行药敏试验,根据药敏试验结果制订化疗方案；选择同期年龄、性别、生存机能状态和手术肿瘤切除程度与试验组相匹配的常规化疗患者为对照组,随访两组的生存机能状态、复发情况和死亡情况。采用SPSS10．0软件进行数据处理。结果试验组评价时的KPS评分为64．52±35．84,对照组则为33．60±36．24,两者存在统计学差异(t=4．516,P=0．000)；术后随访2～4年,试验组的复发率为32．3%,对照组则为60．0%,两者存在统计学差异(X~2=8．620,P=0．003)；试验组的病死率为22．6%,对照组则为48．0%,两者存在统计学差异(X~2=7．978,P=0．005)；试验组的生存率高于对照组,两者存在统计学差异(X~2=7．29,P=0．0069)。结论应用药敏试验指导化疗有助于改善患者的生存机能状态,降低复发率和病死率,提高生存率：药物对肿瘤细胞作用在体外敏感与体内有效间存在密切相关性。更多还原

【Abstract】 Objective To evaluate the correlation between the chemosensitivity of tumor cells in vitro and clinical response in vivo by comparing the difference of curative effect between the chemotherapy of cerebral gliomas directed by chemosensitivity testing in vitro and its routine chemotherapy.Methods Sixty-two patients with cerebral gliomas were recruited as the experimental group,who were subjected to the total resection or subtotal resection of tumor.The cell culture was carried out in vitro after the tumor tissues were resected.Then the chemosensitivity testing was performed by the colorimetric MTT assay.Finally the chemotherapeutic scheme was made according to the result of the chemosensitivity testing.Other 50 patients with cerebral gliomas subjected to the routine chemotherapy were simultaneously recruited as the control group,whose age,gender,survival functional status and operational modes were matched with those of the experimental group.Two groups were equally followed up for the survival functional status,recurrence and death.All data were analyzed using SPSS10.0 software.Results The KPS value in evaluation time was 64.52±35.84 for the experimental group,33.60±36.24 for the control group;there was statistical difference between the 2 groups(t=4.5163,P=0.000).During the follow-up of 2～4 years,the recurrence rate was 32.3% in the experimental group,60.0% in the control group;there was statistical difference between the 2 groups (χ~2=8.620,P=0.003).The mortality was 22.6% in the experimental group,48.0% in the control group;there was statistical difference between the 2 groups(χ~2=-7.978, P=0.005).The recurrence time of the patients in the experimental group was longer than that in the control group,but there was no statistical difference between them.The survival rate of the experimental group was higher than that of the control group,and there was statistical difference between the two(χ~2=7.29,P=-0.0069).Conclusion The direction of chemosensitivity testing in vitro could improve obviously the survival functional status of the patients, decrease the recurrence rate and death rate,and boost the survival rate.The chemosensitivity in vitro has a close correlation with clinical response in vivo更多还原