【摘要】 建立了一种简便、快速、准确检测动物禽流感病毒(AIV)的胶体金免疫层析法(GICA).具体内容包括制备胶体金标记抗AIV单克隆抗体和羊抗AIV抗体,结合垫和样品垫的处理,组装免疫层析试纸条.检测动物AIV,进行敏感性和特异性评定.结果表明:测试条灵敏度可达10 ng/ml.与进口禽流感胶体金免疫层析试剂条比较,符合率达100%.本方法具有特异性强、灵敏度高、简便快速的特点,可用于禽流感的早期诊断.更多还原

【Abstract】 A sensitive,rapid and simple colloidal gold immuochromatography assay(GICA) for detecting AIV of animal sera is developed with the following methods: Colloidal gold was prepared and coupled with the monclonal antibody to AIV,another antibody was goat anti-AIV antibody solidified to Ni-trocellose to prepare a test strip for detecting AIV in sera of animal.Sensitivity and speciality of the strip were identified.The sensitivity of the strip was 10ng/ml.The AIV strip was compared with AIV strip supplied by Yuanh Inc.in Korea.The corresponding rate between the results obtained by using of 2 strip was 100%.This is a specific,simple and rapid assay for measuring AIV and can be used for early diagnosis of AI.更多还原