【摘要】 目的研究转化生长因子β1(TGF-β1)在胆管癌组织中的表达,探讨它与胆管癌生物学行为及预后的关系。方法应用免疫组织化学SP法检测47例胆管癌及癌旁正常胆管组织中TGF-β1的表达,分析其表达与胆管癌临床病理特征的关系,结果进行统计学处理。结果TGF-β1阳性表达主要定位于胆管癌细胞胞浆,偶有胞膜、胞核着色。47例胆管癌组织中TGF-β1阳性表达36例(76.6%),较癌旁正常胆管组织高(P<0.001)。TGF-β1表达与胆管癌临床分期及是否有淋巴结、肝转移有关(P<0.01,0.05,0.05),与组织学分级无关(P>0.05)。结论TGF-β1可能参与了胆管癌的发生、发展。随着胆管癌临床分期的增高而TGF-β1表达增高,其基因缺失可能是胆管癌细胞侵袭性生长转移的重要分子机制,检测TGF-β1表达有助于判断胆管癌的恶性程度及预后。更多还原

【Abstract】 Objective To study the expression of TGF-β1 in cholangiocarcinoma tissues and its biological behavior.Methods TGF-β1 was detected by immunohistochemical methods in 47 paraffin-embedded specimens of cholangiocarcinoma and the control tissues near to the tumor.Results Expression of TGF-β1 was significantly higher in cholangiocarcinoma than that of control tissues near to the tumor(P<0.001).There was significantly relation between TGF-β1 expression and clinical stages,lymph node and liver metastasis(P<0.01,0.05,0.05),and no difference in histological grade.Conclusion Smad4 and TGF-β1,TGF-βRⅡ may play an important role in the occurrence and development of cholangiocarcinoma.Loss of Smad4 and TGF-βRⅡgene may be one of the significant molecular biological mechanism.更多还原