【摘要】 目的:研究三氯化镧(LaCl3)对人肝癌细胞株SMMC-7721生长的影响及其作用机理。方法:体外培养的人肝癌细胞SMMC-7721,给予LaCl3(0.05、0.10、0.50及1.00 mmol.L-1)培养不同时间(1、3、5和7 d),观察癌细胞生长曲线、集落抑制率、培养上清中甲胎蛋白(AFP)的变化。采用流式细胞术检测SMMC-7721细胞周期变化。免疫组织化学检测培养细胞CyclinD1、PCNA、CDK4及P16的表达情况。结果:MTT法显示0.50及1.00 mmol.L-1LaCl3对SMMC-7721生长具有明显的抑制作用,且具有剂量和时间的依赖性;0.10和0.50 mmol.L-1的LaCl3对肝癌细胞集落的形成抑制率分别为51%和67%(P<0.05),1.00 mmol.L-1LaCl3的集落形成抑制率达97%(P<0.01);不同剂量LaCl3作用SMMC-7721细胞3 d后,细胞周期发生明显变化,表现为G0/G1期细胞百分数增加,与对照组比较差异有显著性(P<0.01)。0.10、0.50及1.00 mmol.L-1LaCl3均可抑制AFP的分泌,其中以1.00 mmol.L-1LaCl3组作用最显著(P<0.01)。免疫细胞化学结果显示,LaCl3促进P16蛋白的表达,使Cyclin D1、PCNA和CDK4蛋白的表达下降。结论:LaCl3对SMMC-7721细胞生长具有明显的抑制作用,其机制可能与阻断细胞周期进程、降低细胞的恶性程度有关。更多还原

【Abstract】 Objective To observe the inhibitory effect of LaCl₃ on the growth of human hepatocellular carcinoma cell lines SMMC-7721 and its mechanism.Methods The cells were treated with different doses of LaCl₃ and the growth curve,colony inhibitory rates,the levels of AFP of cultured cells were detected at 1,3,5 and 7 days after they were dealed with LaCl₃.The changes of cell cycle were detected by flow cytometry and the expressions of cell cycle protein CyclinD₁,PCNA,CDK₄ and P16 were observed by immunocytochemistry staining.Results MTT test showed that SMMC-7721 cell proliterating activity was obviously inhibited by LaCl₃ in a time and dose related manner at concentrations of 0.50 and 1.00 mmol·L^-1.The colony forming inhibitory rate of 0.10,0.50 and(1.00 mmol·L^-1)LaCl₃ were 51%, 67% and 97% （P<0.05,P<0.01）.Cell cycle progress of SMMC-7721 changed obviously when treated with different does of LaCl₃,the percent of G₀/G₁ phase was higher and S phase lower,the difference was significant compared with control group（P<0.01）.0.10,0.50 and 1.00 mmol·L^-1 LaCl₃ could decreased the levels of AFP,the effect of 1.00 mmol·L^-1 LaCl₃ was notable（P<0.01）.Immunocytochemistry showed that LaCl₃ could enhance the expression of P16 protein and decrease the expressions of CyclinD₁,PCNA and CDK₄.Conclusion LaCl₃ could inhabit the growth of SMMC-7721 cell and its mechanism might be connected with the inhibition of cell cycle progress and decrease the degree of malignancy.更多还原