【摘要】 甜菜黑色焦枯病毒(Beet black scorch virus,BBSV)新疆分离物(BBSV-X)和宁夏分离物(BBSV-N)分别来自生态环境不同的新疆和宁夏的甜菜产区,自然条件下,BBSV-X含有卫星RNA,BBSV-N不含有卫星RNA。为明确二者的分子差异,以BBSV-X基因组RNA为模板,通过RT-PCR扩增获得了全长cDNA克隆。序列分析显示,BBSV-X基因组RNA全长为3 644个核苷酸,与以往报道的BBSV-N相同,且核苷酸序列一致性高达99.45%。除5′和3′非翻译区各有一个核苷酸发生变异外,核苷酸序列差异主要存在于ORF1通读区和ORF6,氨基酸序列差异仅存在于ORF1的通读区。构建获得了BBSV-X的侵染性cDNA克隆,用体外转录物定量接种了苋色藜(Chenopodiumamaranticolor)和豇豆(Vigna sinensis),结果显示BBSV-X与BBSV-N之间在致病性上存在一定的差异。更多还原

【Abstract】 Two isolates of Beet black scorch virus,BBSV-X and BBSV-N,were isolated from Xinjiang and Ningxia respectively,of which BBSV-X contains a satellite RNA component.To compare their sequence differentiation,full-length cDNA clone of BBSV-X was obtained by RT-PCR.Sequence analysis showed that the genome RNA of BBSV-X consists of 3644 nucleotides in length,same as that of BBSV-N reported previously.It shares 99.45% identity in terms of nucleotide sequence with BBSV-N.Nucleotide differences mainly appeared in ORF₁-RT and ORF₆,while the variation of amino acids only in ORF₁-RT.By inoculation with in vitro transcripts of the cDNAs,the results revealed different pathogenicity between BBSV-X and BBSV-N on Chenopodium amaranticolor and Vigna sinensis.更多还原