【摘要】 目的探讨肿瘤坏死因子-β(TNF-β)+252基因单核苷酸多态性与胃癌病人癌性恶液质的关系。方法105例营养不良胃癌病人,按照近6个月体质量的减轻率分成3组:A组,0~5%;B组,6%~10%;C组,>10%。用PCR结合限制性内切酶检测胃癌病人及110例正常志愿者TNF-β+252单核苷酸多态性。结果TNF-β+252A(TNFB2)出现的频率在胃癌组和对照组差异无显著性(P>0.05)。在胃癌病人中,TNFB2出现的频率在A、B、C各组差异亦无显著性(P>0.05);恶液质病人TNFB2出现的频率与对照组比较差异无显著性(P>0.05)。结论TNF-β+252基因单核苷酸多态性与胃癌恶液质的发生无相关性。更多还原

【Abstract】 Objective To evaluate the association of TNF-β+252 mononucleotide polymorphisms with dyscrasia related to gastric cancer.MethodsThis study was carried out in 105 gastric cancer patients with malnutrition who were divided into three groups according to their weight loss in the last six months: group A,weight loss of 0-5%;group B,6%-10%;and, group C,>10%.The control group consisted of 110 healthy volunteers.The mononeucleotide polymorphisms study was done by Nco Ⅰ-digested DNA fragment biding PCR.ResultsNo significant differences were found in TNFB2 frequency between the patients and the controls(P>0.05),and between dyscrasia group and controls(P>0.05).No significant difference was seen in(TNFB2) frequency among group A,B and C(P>0.05).ConclusionTNF-β+252 polymorphism is not associated with the pathogenesis of severe dyscrasia.更多还原