【摘要】 目的观察卵巢切除大鼠脑内不同部位胆碱乙酰化转移酶(ChAT)阳性细胞表达的变化,了解ChAT改变与轻度认知障碍(MCI)相关的可能机制。方法成年健康雌性Wistar大鼠14只,应用SINGH法建立大鼠卵巢切除模型(模型组),对照组除不切除卵巢外,其余步骤同模型组。常规处死大鼠,取脑组织行ChAT免疫细胞化学染色,观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核等部位ChAT阳性细胞表达变化。结果模型组与对照组比较脑内海马CA1区、大脑皮质区、杏仁复合体区、Meynert核区ChAT阳性细胞数明显减少(t=5.15~16.48,P<0.01)。结论雌激素下降可致大鼠脑内多部位ChAT阳性细胞表达减少,这种病理变化可能与MCI发病有关。更多还原

【Abstract】 Objective To observe the changes of choline acetyltransferase(ChAT) in different brain sections of rats after ovariectomy. Methods Fourteen Wistar rats were divided into two groups: model group(rats with ovariectomy),and control group(no ovaries removed).The brain tissues were taken out and immunoreactive cells of ChAT were observed by using immunohistochemical technique in CA1 section of hippocampi,cortex section,nucleus amygdalae section and nucleus of Meynert.The amount of immunoreactive cells of ChAT was compared in different areas between the two groups. Results The immunoreactive cells of ChAT remarkably decreased in hippocampus CA1,cerebral cortex,nucleus amygdalae and Meynert nucleus of the brain in the model group than in the control group(t=5.15-16.48,P<0.01). Conclusion The reduction of estrogen in the body can cause the reduction of ChAT in some brain sections,and this pathological variation may be related to the occurrence of mild cognitive impairment.更多还原