【摘要】 目的探讨肺癌信号传导及免疫相关基因与正常肺组织差异表达。方法采用包含1152个cDNA克隆的基因芯片技术,分别对4例肺鳞癌组织及相对应的正常肺组织进行信号传导及免疫相关特异性基因表达谱的比较。结果3例以上共同表达的差异基因78条,其中上调者共44条,下调者共34条。低表达基因主要集中在蛋白质和脂肪等分子的代谢及趋势化因子等方面。高表达的基因包括细胞表面受体、核受体和离子通道等有关信号机制、cAMP、蛋白激酶及蛋白磷酸化酶的作用、细胞凋亡和蛋白降解等。结论细胞信号传导相关基因在肺癌中表达有较大的个体差异,与癌细胞分化程度有关,生物信号传导及肿瘤免疫有关的基因的差异表达分析,可以了解肺癌的发生机制,为今后的研究工作提供线索。更多还原

【Abstract】 Objective To investigate signal transmission or immune related gene expression profiling in the tissue of lung squamous carcinoma.Methods Samples of 4 lung squamous carcinoma and distal normal lung tissues were obtained from pulmonary lobectomy.Signal transmission or immune related gene expression were mapped by a DNA array of 1152 ordered cDNAs.Results They were detected 44 over expression and 34 down expression genes in at least 3/4 of the samples.Down expression genes mainly focused on the metabolism enzyme of protein and fatty,but over expression genes wereinvolved in cell surface and nuclear receptor,ion channel,cAMP,protein kinas,phosphorylase,protein degradation or cell apoptosis.Conclusion Signal transmission or immune related gene expression profiling of lung squamous carcinoma differed individually and may related to the tumor cellular differentiation.This study not only helps to clarify the pathogenesis of lung cancer but also to develop new strategies for signal transmission pathway research of cancer tissues.更多还原