【摘要】 用二苯基苦基苯肼(DPPH)自由基酶标仪法,对拟青霉属5个种的20个菌株培养液提取物的自由基清除活性进行了比较研究,发现不同种拟青霉的甲醇提取液的自由基清除活性差异很大,在浓度为5.0 mg.mL-1,37℃保温10 m in时,自由基清除活性最高为71.9%,最低为15.9%;同种不同菌株之间的活性也有较大的差异。自由基清除活性均与反应时间和样品浓度成正相关关系。二苯基苦基苯肼自由基薄层实验结果表明,该属菌株培养液提取物中自由基清除剂都不是单一成分,且组成也不完全相同。更多还原

【Abstract】 A DPPH-microplate assay and DPPH-TLC assay were used to test the free radical scavenging activities of methanol extracts from 20 strains belonging to 5 species of Paecilomyces.The results revealed that the free radical scavenging activities of the methanol extracts were different.The highest free radical scavenging rate is 71.9%,while the lowest is 15.9% at the concentration of 5.0 mg·mL^-1 after incubated at 37 ℃ for 10 min.We also found that the free radical scavenging rates of all the tested fungi are positively related to their concentrations and reaction time.Meanwhile,the DPPH-TLC assays showed that the components of extracts from different strains were different,and all the strains contain more than one free radical scavenger.更多还原