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7株细脚拟青霉代谢物中抗真菌活性物质的初步研究
Preliminary report on anti-fungi activity of metabolites from seven Paecilomyecs tenuipes strains
【摘要】 以1种霉菌为指示菌,采用薄层色谱法对7株细脚拟青霉发酵的代谢产物进行抑菌活性试验。根据抑菌圈大小判定代谢物抑菌活性的大小,从而筛选出高活性菌株。同时以致病菌白色念珠菌为指示菌,采用牛津杯法对筛选出的菌株进行了进一步抑菌活性验证,确认了RCEF0969菌株具有较高的抑制真菌的活性,其薄层色谱法的抑菌圈直径为22.65 mm。
【Abstract】 An anti-fungi TLC assay was employed to screen antimicrobial metabolites from the selected entomogenous fungi strains of Paecilomyces tenuipes.Meanwhile,an anti-Candida.albicans Oxford Cup assay was made to confirm their anti-fungal activities.The results revealed that the strain RCEF0969 of Paecilomyces tenuipes has the strongest anti-fungi activity with a 22.65 mm diameter of inhibition zone.
【关键词】 虫生真菌;
代谢物;
薄层色谱;
抑菌;
细脚拟青霉;
【Key words】 entomogenous fungi; metabolite; thin layer chromatography; inhibition zone; Paecilomyces tenuipes;
【Key words】 entomogenous fungi; metabolite; thin layer chromatography; inhibition zone; Paecilomyces tenuipes;
【基金】 国家自然科学基金项目(30570189);安徽省自然科学基金项目(00042425号)共同资助
- 【文献出处】 安徽农业大学学报 ,Journal of Anhui Agricultural University , 编辑部邮箱 ,2006年04期
- 【分类号】S476
- 【被引频次】13
- 【下载频次】213