【摘要】 对诱变菌株绿色木霉F-UV264产纤维素酶条件进行了研究,结果表明:以1%(NH4)2SO4和2%酵母膏为氮源,并加入3%表面活性剂,2%Mandels营养盐,麸皮与稻草粉比例为1∶3,加水量为干料的4倍,可提高酶的产量。该菌株最适产酶培养条件为pH值4.6~5.6,30℃条件下发酵96~135 h纤维素酶的酶活性达到较高水平。此研究可为工业化生产纤维素酶提供依据。更多还原

【Abstract】 In this paper,the producing cellulase conditions of mutant stain Trichoderma F-UV₂₆₄ were stduied.The result of experiment showed that: the optimal nitrogen source is 1%（NH₄）₂SO₄ and 3%the surface active material,the optimal sult group is 2%Mandels,bran:straw is 1∶3,solid:liquid is（1∶4）.The enzyme vitality of cellulase can be increased after adding these influental factors.The optimum culture condition of producing cellulase is pH（4.6）～5.6 at 30 ℃ for 96～135 h,and the maximum cellulase activity is reached.This research can provide the basis for the cellulase manufacture.更多还原