【摘要】 采用超声技术提取中药刺五加中的乌索酸和齐墩果酸,并建立HPLC-PAD法测定了乌索酸和齐墩果酸的含量。色谱柱为Nova-Pak C18(3.9 mm×300 mm,4μm),流动相为甲醇∶水(体积比,88∶12),流速为0.8 ml/min,二极管阵列检测器检测,检测波长210 nm,柱温25℃。乌索酸线性范围为0.136~1.224μg,线性回归系数为0.999 5;齐墩果酸线性范围为0.052~0.468μg,线性回归系数为0.999 4。样品中乌索酸的平均回收率为98.1%,RSD为1.1%(n=5);齐墩果酸的平均回收率为97.5%,RSD为1.0%(n=5)。该方法操作简便,结果准确、可靠,可用于刺五加中乌索酸和齐墩果酸含量的测定分析。更多还原

【Abstract】 Ursolic acid and oleanolic acid in Acantopanax senticosus(Rupr.et Maxim.)Harms were extracted with the ultrasonic technology.A determination method of ursolic acid and oleanolic acid was established with the high performance liquid chromatograph and photodiode array detector.The chromatographic column,Nova-Pak C18(3.9?mm×300?mm,4?μm),methanol-water(88∶12,V/V),mobile phase with 0.8 ml/min flow rate,the detected wave length(210?nm) and the column temperature(25 ℃) were adopted.The calibration curve of ursolic acid was linear under the content of 0.136～(1.224)?μg and the correlation coefficient were 0.999?5.The calibration curve of oleanolic acid was linear under the content of 0.052～0.468?μg and the correlation coefficient were 0.999?4.The average recovery of ursolic acid was 98.1% and RSD was 1.1%(n=5).The average recovery of oleanolic acid was 97.5% and RSD was 1.0%(n=5).The content determination of HPLC was accurate and reliable and can be used to determine the content of ursolic acid and oleanolic acid in ?Acantopanax senticosus ?Harms.更多还原