【摘要】 目的:观察病毒性心肌炎(VMC)T细胞凋亡与凋亡蛋白表达的变化以及清心胶囊对这一环节的干预作用。方法:复制VMCBALB/c小鼠模型,给予清心胶囊治疗后,采用光镜和透射电镜观察心肌病理改变,Annexinv/PI双染色流式细胞术和透射电镜观察T淋巴细胞凋亡;流式细胞术检测Fas、FasL、bcl-2及Bax蛋白在T细胞上的表达。结果:(1)模型组小鼠心肌病变明显,治疗组心肌病理改变明显减轻,正常组心肌无明显病理改变。(2)与正常组比较,VMC小鼠脾T细胞的凋亡率均显著升高(P<0.01);与模型组比较,治疗组小鼠脾T细胞的凋亡率均显著降低(P<0.01),但与正常组比较,仍有显著性差异(P<0.01)。透射电镜下可见正常T细胞和凋亡T细胞的典型形态,模型组T细胞凋亡增多。(3)与正常组比较,模型组小鼠脾T细胞Fas、FaSL表达均显著升高(P<0.01)lbcl-2表达均显著降低(P<0.01);与模型组比较,治疗组小鼠脾T细胞FasL表达均显著降低(P<0.01),bcl-2表达均显著升高(P<O.01、),Fas表达无统计学差异(P>0.05);与正常组比较,治疗组小鼠脾T细胞FaSL表达无显著性差异(P>0.05),Fas、bcl-2表达仍有显著性差异(P<0.05);而Bax表达各组间均无显著性差异(P>0.05)。结论:VMC发病过程中存在T细胞过度凋亡现象,这与Fas、FasL表达升高和bcl-2表达降低有关;清心胶囊能够显著抑制T细胞的过度凋亡,与其下调FasL表达和上调bcl-2表达有关。更多还原

【Abstract】 Objective: To investigate the changes of T Lymphocyte Apoptosis and expression of apoptotic protein in the mouses with viral myocarditis, and interference effect of QingXin capsule on them. Methods :BALB/c mouses with VMC were established, after treated with QingXin capsule, the cardiac pathological changes were observed by light microscope and trans-misson electron microscope, apoptosis of T lymphocytes were examined by Annexin v/PI double stain flow cytometry and transmisson electron microscope, expression of Fas, FasL, bcl-2 and Bax on T lymphocytes were detected by flow cytemetry. Results: (1) The myocard pathological changes of mouses in model group are more serious than those in treated group, there are no myocard pathological changes in normal group. (2) Apoptosis percentage of spleen T lymphocyte of mouses with VMC are higher than those in normal group(P<0.01), comparing with model group, apoptosis rates of spleen T lymphocyte of mouses in treated group go down significantly(P<0.01), but there still are marked statistical differences in those between treated group and normal group(P<0.01).Through transmisson electron microscope, we can see typical appearance of the normal T lymphocytes and apoptotic T lymphocytes, moreover, there are more apoptotic T lymphocytes in model group than those in other groups.(3)Comparing with normal group, expression of Fas and FasL on T lymphocytes of mouses with VMC goes up significantly(P < 0.01), expression of bcl-2 on them goes down significantly(P< 0.01). Comparing with model group, expression of FasL on T lymphocytes of mouses in treated group goes down significantly(P < 0.01), expression of bcl-2 on them goes up significantly(P<0.01), but there are no statistical differences on expression of Fas on T lymphocytes between treated group and model group(P>0.05). there are no marked changes of Bax expression among groups(P<0.05). Conclusions: There exsits over-apoptosis of T lymphocytes in acute stage of VMC, and it is revealed that apoptosis process is associated with upregulation of Fas, FasL expression and downregulation of bcl-2 expression. Qing-xin capsule can significantly inhibit over-apoptosis of T lymphocytes in associated with its effects that it makes expression of FasL downregulated and expression of bcl-2 upregulated.更多还原