【摘要】 目的探讨奥曲肽对肝癌微血管密度(MVD)和血管内皮生长因子(VEGF)表达的影响。方法分别于16只裸小鼠右腋窝皮下注射HepG2细胞建立动物模型后,随机分为2组,每组各8只。实验组:皮下接种肿瘤1d后给予奥曲肽[100μg/(kg·d)]腹腔注射,连用14d;对照组:予以相同容积的无菌生理盐水腹腔注射,连用14d。14d后处死小白鼠,检测肿瘤大小、血管密度(MVD)及血管内皮生长因子(VEGF)。结果皮下接种7d后实验组肿瘤的体积均明显小于对照组(<0.01)。实验组MVD、VEGF表达明显低于对照组(P分别<0.01和0.05)。结论奥曲肽对裸小鼠肝癌移植瘤具有抑制作用,对肿瘤MVD及VEGF的抑制作用可能为其机制之一。更多还原

【Abstract】 [Objective] To evaluate the effect of 0ctreotid on the expression of MVD andVEGF in seeding carcinoma. [Meghods] To inject 0.2 mL HepG2 cells into subcutaneous tissue at right axilla for preparation animal mode respectively in 16 nude mice,and then randomized into two groups: Experimental and control group, 8 mice each group. Experimental group: Every one received octreotide (100 μg/(kg·d)×14 days) intraperitoneal injection after implantation. control group: each mouse received same amount of saline solution intraperitoneal injection. Subcutaneous tumor’s size was measured everyday. All mice were killed on 14d after implantation, and the tumors werere moved for immunohistochemical examination of MVD AND VEGF. [Results] Mean volum of tumors was smaller in experimental group than in control group after 7 days (0.01). The expression of MVD and VEGF was significantly lower in experimental group than in control group (P <0.01 and 0.05 respectively). [Conclusion] Octreotid can inhibit seeding tumor of HepG2 cells in nude mice. Inhibition of tumor angiogenesis maybeone of the mechanisms of its antitumor effect by inhibition of MVD andVEGF.更多还原