【摘要】 利用cDNA-AFLP技术分析了授粉前后黄瓜幼果的基因表达差异。有64条转录本片段(TDF)出现在授粉后6~72h的黄瓜幼果中,其中5条经反向Northern斑点杂交证实主要在授粉后的幼果组织中表达。序列分析确定,有一条TDF属于编码黄瓜扩张蛋白的新基因,命名为Cs-Expansin10(CsExp10),可能与授粉后黄瓜果实膨大生长相关;另一条与谷胱甘肽还原酶基因高度同源;其余3条在基因库中未找到相似序列。更多还原

【Abstract】 Differential expression of genes inyoung fruit of cucumber (Cucumis sativus L.)before/after pollination was investigated viacDNA amplified fragment length polymorphism(cDNA-AFLP). There were 64 transcript-derivedfragments (TDFs) displayed specially in youngfruits after pollination of 6–72 h (Table 1), fiveof which were verified to be mainly expressedin young fruits by reverse Northern dot blotting(Fig.2). Sequence data showed one TDF was anew gene encoding expansin and was named asCs-Expansin10 (CsExp10), which might be re-lated to the expanding growth of cucumber fruit after pollination (Fig.3), another was signifi-cantly similar to the glutathione reductase gene,and the others had no similar sequence inGenBank database (Table 2).更多还原