【摘要】 研究了分光光度法测定盐霉素含量的方法。检测波长为528nm,以95%乙醇为溶剂,4%香草醛溶液为显色剂,显色温度70～74℃,显色时间40min。盐霉素浓度在40～280u/mL范围内与吸光度呈线性关系,相关系数0.9996(n=6),回收率在100.1%～100.8%(n=5);RSD在0.8%～1.2%。本法适用于制药厂盐霉素生产的中间体质量控制。更多还原

【Abstract】 A spectrophotometric method for quantitative analysis of salinomycin was proposed. Salinomycin was dissolved in ethanol, and 4% vanillin solution was used as chromogenic agent at the temperature from 70 ℃ to 74 ℃ about 40 minutes, salinomycin derivative was formed. It can be measured at 528 nm on visible spectrophoto- meter . Salinomycin presented a good linear relationship between its concentration and absorption value in the range of 40~280 u/mL with r=0.999 6(n=6). The average recovery was between 100.1% and 100.8%(n=5), and RSD was from 0.8% to 1.2%.更多还原