【摘要】 【目的】研究巨噬细胞移动抑制因子(macrophagemigrationinhibitoryfactor,MIF)和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)在鼻咽癌组织中的表达水平及其与肿瘤新生血管的关系,探讨细胞因子在鼻咽癌细胞侵袭转移过程中的作用。【方法】收集1999-2003年期间45例确诊未治的鼻咽原发癌活检组织标本,用LSAB免疫组化法检测鼻咽癌组织中MIF和VEGF表达,计数癌组织中新生血管热区的CD34阳性微血管密度(intratumoralmicrovesseldensity,IMD),并将各检测指标与患者的病理及临床资料相联系。【结果】鼻咽原发癌组织中,癌细胞MIF和VEGF的阳性表达率分别为78%(35/45)和71%(32/45),伴有淋巴结转移的癌组织中MIF和IMD水平均高于无淋巴结转移的癌组织,P =0.029,P=0.026,MIF阳性组的IMD计数为(35.1±13.3)/高倍视野,明显高于MIF阴性病例的(20.9±10.2)/高倍视野,P=0.003。以Schmincke型生长方式分布的癌细胞MIF表达水平(67.4%±35.2%)高于以Regaud型方式分布的癌细胞(32.9%±29.7%),P=0.007。癌细胞MIF与VEGF表达以及IMD计数均显示正相关关系,P<0.05。但癌细胞VEGF的表达与患者性别、年龄、病理组织学分型以及临床分期无统计学显著意义。【结论】MIF在鼻咽癌细胞的淋巴结转移过程中可能具有重要的促进作更多还原

【Abstract】 To detect the expression of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF) in nasopharyngeal carcinoma (NPC), and correlate their expression level with intratumoral microvessel density (IMD) to investigate the mechanism of invasion and metastasis of NPC cells. Forty-five NPC biopsies were collected from the patients with NPC during 1999-2003. Immunohistochemical staining was used for detecting the expression of MIF, VEGF in NPC tissues. The number of IMD was evaluated by counting the CD34-positive neovessels in the most active areas of tumor neovascularization or hotspots. The associations of these indexes with pathological and clinical features were statistically analyzed. The positive expression rates of MIF and VEGF were 78% (35/45) and 71% (32/45), respectively. The expression of MIF showed a correlation with lymph node metastasis. The higher expression of MIF were always found in NPC neoplastic cells with cervical lymph node metastasis than that in NPC without lymph node metastasis (P =0.029). The number of IMD in tumor with metastasis was also significantly higher than that without lymph node metastasis (P =0.026). Meanwhile, the number of IMD counted in MIF positive group (35.1±13.3/HPF) was significantly higher than that in MIF negative group (20.9/HPF±10.2/HPF), P value was 0.003. In addition, the expression of MIF in NPC neoplastic cells with Schmincke growth pattern (67.4%±35.2%) was higher than that in NPC with Regaud growth pattern (32.9%±29.7%), there existed a significantly difference in MIF expression of NPC between two growth patterns (P =0.007). There were positive correlations found for the expression of MIF with VEGF and IMD counting, respectively (P < 0.05). However, there was no relationship found in the expression of VEGF with the other clinicalopathological features of NPCs including age, gender, histopathological type, and clinical stage. [Conclusion]MIF cytokine might play an important promoting role in contributing the metastasis of NPC as well as associated with tumor neovascularization and up-regulating the VEGF expression of NPC.更多还原