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当归芍药散防治老年期痴呆的物质基础与作用机理研究V——FBD多糖的组成
Study on Substance Basis and Mechanism of Action of Dangguishaoyaosan in the Prevention and Treatment of Senile Dementia V:Constitutes of Polyhexoses from FBD
【摘要】 目的:研究当归芍药散精简方FBD多糖的组成。方法:提取和精制FBD多糖及其组成药材茯苓、白术及当归中多糖,采用SephadexG75、G150凝胶柱分离纯化各多糖样品;HPLCELSD法测定各多糖平均分子量;GC法测定多糖的单糖组成。结果:经凝胶柱分离得到三个多糖PsA、PsB、PsC,分子量分别为1777577、400172、4369;PsA由甘露糖、果糖组成;PsB由甘露糖、葡萄糖、果糖组成;PsC由鼠李糖、甘露糖、果糖组成。结论:首次报道并确定了FBD多糖部位的组成。
【Abstract】 Object:To study chemical constituents of polyhexoses fraction from FBD.Methods:FBD polyhexoses were segregated and purified with Sephadex G-75、G-150.Their molecular weights were measured with HPLC-ELSD;The components and proportions of monosaccharides were hydrolyzed and analyzed with GC.Results:We obtained polyhexoses PsA、PsB、PsC from FBD and measured their molecular weights as 1777577,400172,4369.PsA consisted of Rhamnose and fructose,PsB consisted of Rhamnose、glucose and fructose,PsC consisted of Rhamnose、mannose and fructose.Conclusion:We determined the chemical constituents of polyhexoses fraction from FBD.
【Key words】 FBD; Dang-gui; Bai-shu; Fu-ling; polyhexose; HPLC; GC;
- 【文献出处】 中国实验方剂学杂志 ,Chinese Journal of Experimental Traditional Medical Formulae , 编辑部邮箱 ,2005年04期
- 【分类号】R285.5
- 【被引频次】7
- 【下载频次】510