【摘要】 目的探讨尿激酶区域动脉灌注(LAI)对重症急性胰腺炎(SAP)大鼠微循环的影响。方法将1 2 0只大鼠随机分成4组,每组4 0只。A组为假手术组(S组);B组为SAP组;C组为SAP尿激酶LAI组;D组为SAP生理盐水LAI组(对照组)。以激光多普勒检测大鼠胰腺血流量,以伊文思蓝(Evans B lue)漏出率检测胰腺微循环血管的通透性,同时观察胰腺病理变化。结果(1)胰腺血流量(PBF):A组1 h和3 h的胰腺PBF高于其他3组,差异有显著性(均P<0.0 5);B组1 h和3 h的PBF与D组差异无显著性;C组1 h和3 h的PBF明显高于B组和D组(P<0.0 5)。(2)EB漏出率:B,C,D组胰腺组织EB的漏出率显著高于A组(均P<0.0 5),但B,C,D组之间无统计学差异(均P>0.0 5)。(3)胰腺组织病理评分:术后1,3 h时,C组评分明显高于A组(P<0.0 5),但明显低于B,D组(均P<0.0 5),B,D组之间无明显差异(P>0.0 5)。结论尿激酶LAI能改善SAP大鼠胰腺微循环,但不能完全阻止SAP的病理演变。更多还原

【Abstract】 Objective To investigate the effects of regional arterial infusion of urokinase(LAI) on the(pancreatic) microcirculation in rats with severe acute pancreatitis(SAP).Methods Rats were divided into 4 groups: sham operation(A) group,SAP(B) group,urokinase LAI(C) group and normal saline LAI(D) group.Pancreatic blood flow was measured by laser Doppler instrument,pancreatic microvascular permeability was determined by measurement of extravasation of Evans Blue,and pathological changes of pancreas were(observed).Results(1)Pancreatic blood flow(PBF):au 1h and 3h,PBF in A group was significantly(higher) than that in B,C,D group(all P<0.05),but there was not significant difference between B and D group(P>0.05);however,PBF in C group was significantly higer than that in B and D group(P<(0.05)).(2)Extravasation of Evans Blue:in normal pancreat tissue was(149.11±17.93)ug/g,which was significantly lower than that in B,C,D group((all P>0.05),but there was not obvious difference(between) B,C,D group(all P>0.05).(3)Pathological score of C group at 1h and 3h was significantly higher than the A group(P<0.05),but was significantly lower than that of B and D group(P<(0.05));howerer there was not significant difference between B and D group(P>0.05).Conclusions (Urokinase) LAI can improve the microcirculation of rats with SAP,but can not completely prevent the(pathological) changes of SAP.更多还原