【摘要】 目的探讨基质细胞衍化因子-1(SDF-1)在不同细胞系及乳腺癌组织和正常乳腺组织中的表达,并分析SDF-1的表达水平与乳腺癌患者预后的关系。方法选择乳腺癌细胞系、成纤维细胞系、血管内皮细胞系,利用RT-PCR方法分析SDF-1mRNA的表达。采用免疫组织化学染色和实时定量PCR测定120例乳腺癌组织和32例正常乳腺组织中SDF-1的表达。通过SPSS统计软件对所得结果行统计学分析。结果SDF-1的表达见于人胚胎肺成纤维细胞系、某些乳腺癌细胞系(MDA-MB435s、MDA-MB436、MCF7)及乳腺癌和正常乳腺组织。淋巴结有转移者SDF-1mRNA的表达水平为399.00±210.00,淋巴结无转移者为0.89±0.47(P=0.048)。预后不良者(出现肿瘤局部复发、远处转移和因乳腺癌死亡者)较无疾病存活者SDF-1mRNA水平明显增高,为670.00±346.00vs0.83±0.35(P=0.01)。SDF-1mRNA的表达水平与患者的总存活率和无瘤存活率有关,P值分别为0.01和0.035。结论SDF-1mRNA的表达既可见于基质细胞也可见于乳腺癌细胞。SDF-1的表达水平与乳腺癌患者有无淋巴结转移、预后和存活密切相关。SDF-1可作为乳腺癌患者预后判断的一项指标。更多还原

【Abstract】 Objective Stromal cell-derived factor-1(SDF-1, CXCL12) is a member of the CXC subfamily of chemokines which, through its cognate receptor (CXCR4), plays an important role in tumor invasion and metastasis. This study analyzed quantitatively the expression of SDF-1 and its relation with clinicopathologic feature and clinical outcome in human breast cancer.Methods Expression of SDF-1 mRNA in 8 breast cancer cell lines, an endothelial cell line HECV and a fibroblast cell MRC5 was studied by using RT-PCR. In addition, the expression of SDF-1 was investigated at both protein (immunohistochemistry) and mRNA(real-time PCR) levels in a group of human normal mammary(n=32) and tumour tissues(n=120). Results SDF-1 expression was identified in MRC5, MDA-MB435s, MDA-MB436, MCF7 cell lines, breast tumour and normal tissues. Significantly higher level of SDF-1 was seen in lymph node positive than in lymph node negative tumours (399.00±210.00 vs 0.89± 0.47), P= 0.048. The level of SDF-1 expression in patients who developed local recurrence or metastasis, or patients who died of breast cancer was higher than in patients who were disease free as well, (670.00±346.00 vs 0.83±0.35), P=0.01. It was most notable that level of SDF-1 was significantly correlated with over survival (P=0.01) and incidence free survival (P=0.035, by Cox proportion analysis).Conclusion SDF-1 is a factor that is expressed in both stromal cells and some breast cancer cells. Its level are correlated with lymph node involvement, prognosis and survival in patients with breast cancer. SDF-1 may therefore have a potential prognostic value in breast cancer.更多还原