【摘要】 目的建立一种RP-HPLC测定珍菊降压片中氢氯噻嗪和芦丁的含量。方法色谱柱为Penomenex C18反相柱(250 mm×4.6 mm,5μm),流动相为0.05 mmol.L-1磷酸二氢钾溶液-乙腈(80∶20,pH 3.0),检测波长为271 nm,柱温为35℃,流速为1.0 mL.min-1,采用外标法定量。结果氢氯噻嗪和芦丁的线性范围分别为10.06~100.6μg.mL-1(r=0.999 9)和40.08~400.8μg.mL-1(r=0.999 9),平均回收率分别为99.86%和99.70%(n=9);精密度良好。结论本法简便、灵敏、准确,适于珍菊降压片中氢氯噻嗪和芦丁的质量控制。更多还原

【Abstract】 OBJECTIVE To establish a method for the determination of hydrochlorothiazide and rutin in Zhenjujiangya tablets by RP-HPLC.METHODS HPLC was used with Penomenex C₁₈（250 mm×4.6 mm,5 μm） column,0.05 mol·L^-1sodium dihydrogen phosphate solution-acetonitrile（80∶20,pH 3.0） was used as the mobile phase.The detection wavelength was 271 nm and the flow rate was 1.0 mL·min^-1.RESULTS The linear response range was 10.06～100.6 μg·mL^-1for hydrochlorothiazide（r=0.999 96）and 40.08～400.8 μg·mL^-1for rutin（r=0.999 93）,respectively.The average recoveries of hydrochlorothiazide and rutin were 99.86% and 99.70%（n=9）respectively,with good precision.CONCLUSIONS The method is quick,sensitive,accurate and suitable for the determination of hydrochlorothiazide and rutine in Zhenjujiangya tablets.更多还原