【摘要】 目的:研究肿瘤坏死因子- α(TNF- α)在病毒性心肌炎(VMC)发病中的作用及临床意义。方法:在建立VMC动物模型的基础上,用ELISA法检测VMC小鼠血清TNF- α的变化,同时用TNF- α单克隆抗体(mAb)和重组TNF- α(rTNF- α)进行干预治疗,比较干预组和对照组小鼠死亡率和心肌病变。结果:VMC小鼠血清TNF -α水平明显升高,VMC心肌炎细胞浸润、坏死与TNF -α的水平有关。TNF -α干预组小鼠死亡率增加,心肌炎细胞浸润增加,心肌坏死加重;而TNF -αmAb干预组小鼠死亡率明显减低,TNF -αmAb能减轻VMC的炎细胞浸润和心肌坏死。结论:TNF -α参与了VMC的免疫反应和发病机制,这对VMC的治疗有重要的指导意义。更多还原

【Abstract】 Objective:To study the effect and clinical significance of tumor necrosis factor-α(TNF-α) in viral myocarditis(VMC).Methods:Animal model was established by serum TNF-α in VMC mice was assayed by the way of ELISA.In addition,VMC mice were treated by TNF-α mAb and rTNF-α,death rate andmyocardial change of intervention group were compared with control group.Results:Serum TNF-α in VMC mice increased apparently.Inflammatory cell infiltration and myocardial necrosis had ralations with TNF-α in VMC.Inflammatory cell infiltration and myocardial necrosis in the mice of exogenous TNF-α intervention group were more serious than simple VMC group apparently,death rate of mice was also higher than simple VMC group.Inflammatory cell infiltration and myocardial necrosis in the mice of TNF-α mAb intervention group were milder than simple VMC group apparently,death rate of mice was also lower.Conclusion:TNF-α take part in the immunoreaction and pathogenesis of VMC,it has important significance to the therapy of VMC.更多还原