【摘要】 目的:探讨小鼠胃内接种BCG后,细菌在体内的定植及机体能否产生针对结核杆菌的免疫力。方法:在小鼠胃内接种BCG的第15、30、6 0天,检测结核杆菌在小鼠体内脏器的定植;接种BCG的第6 0、90天,检测小鼠脾淋巴细胞受到PPD刺激后转化率、小鼠脾淋巴细胞受到PPD刺激后IL 2表达量。结果:小鼠的肠系膜淋巴结和脾脏中有结核杆菌定植;淋巴细胞的转化率分别为31. 32 % ,37 .94 % (与皮内接种无显著性差异,与未免疫组有显著性差异) ;脾淋巴细胞IL 2表达量分别为37 4 7±5 . 6 0U ml,39. 4 1±7 .73U ml(与皮内接种无显著性差异,与未免疫组有显著性差异)。结论:胃内接种BCG可以产生与皮内接种一样的抗结核免疫效力。更多还原

【Abstract】 Objective:To determine the location of mycobacterium tuberculosis(MTB) and whether the cellular immunity is induced after intragastric administration of BCG in mice.Methods:Viable MTB were detected in mesenteric lymph nodes?spleen and lungs at day 15,30 and 60 after intragastric administration of BCG to mice.The proliferation of T-lymphocyte with the stimulus of the purified protein derivative(PPD) was measured at day 60 and 90 after intragastric administration of BCG to mice.The expression of interleukin-2(IL-2) of T-lymphocyte in spleen was detected at day 60 and 90 after intragastric administration of BCG.Results:Viable MTB were detected in the mesenteric lymph nodes and spleen at day 15,30 and 60 after intragastric administration.At day 60 and 90 after intragastric administration of BCG to mice,the ratio of T-lymphocyte proliferation was 31.32%,37.94% separately and the production of IL-2 was 37.47±5.60 U/ml,39.41±7.73 U/ml separately.There were no statistically significant differences in the cellular immunity between the mice of the intragastric administration of BCG significant differences in the cellular immunity between the mice of the intragastric administration and the mice of the subcutaneous vaccination of BCG( P <0.01),while there were statistically of BCG and the no-immunization mice( P >0.05).Conclusion:Intragastric administration of BCG can induce cellular immunity in mice.更多还原