【摘要】 目的更深入了解人食管鳞状细胞癌中p53基因突变的地域差异及临床特征之间的差异。方法从中国食管鳞癌的高发区河南林县以及浙江省收集共92例人食管鳞状细胞癌(ESCC)标本,使用免疫组化、PCR-SSCP分析和DNA测序的方法分析p53肿瘤抑制基因。结果p53基因的突变或过表达分别为30.4%和51.1%(P<0.05)。p53的过表达与肿瘤的转移和患者存活期相关,两个不同地域收集的肿瘤标本中发现了不同的突变模式。结论p53的突变或过表达在食管鳞癌的发展中可能起到了重要的作用。致癌因子的各种不同组合,可能是两个地区人群中基因组的不稳定性所致。这种不稳定性在某种水平上决定了不同地区不同的发病率。更多还原

【Abstract】 Objective To elucidate further the clinical characteristic and regional difference of p53 gene mutation in human esophageal squamous cell carcinoma. Methods Immunohistochemistry, PCR SSCP, DNA sequencing were employed to analyse p53 gene in 92 esophageal carcinoma samples collected from Linxian of Henan province and Zhejiang province, the high incidence areas in China for esophageal carcinoma. Results The over expression rate of p53 was 51.1 %, which related to tumor metastasis and survival time. The mutation rate of p53 was 30.4 %, (P <0.05). Different mutation types were found in the carcinoma samples from two areas. Conclusions Mutation and over expression of p53 may play an important role in esophageal squamous cell carcinoma occurrence and progression. Different combination of carcinogen, which resulted from genome instability in population, determined different incidence rate in the two areas.更多还原