【摘要】 目的　研究卵巢上皮癌细胞中血管内皮生长因子 (VEGF)、血管内皮生长因子受体(VEGFRs)的表达与血管内皮细胞内的信号转导和转录活化因子 (STATs)的活化及其相关性 ,并阐明VEGF对卵巢癌细胞的直接作用及机制。方法　选择 4 2例卵巢上皮性癌患者作为研究对象 ,2 9例卵巢良性肿瘤患者和 11例正常卵巢组织作为对照 ,用免疫组织化学LSAB法检测组织中VEGF、VEGFRs、STATs蛋白的表达 ,并进行半定量及相关性分析。结果　 (1)VEGF定位于胞浆 ,VEGFRs定位于胞浆和胞膜。VEGF在卵巢癌细胞呈高表达 ,VEGFRs在卵巢癌细胞及间质血管内皮细胞中均呈高表达 ,与良性及正常对照组差异有统计学意义 (P <0 .0 1)。 (2 )P STATs定位于胞浆和胞核。在卵巢癌细胞及血管内皮细胞中 ,P STAT3和P STAT5高表达 ,与两对照组相比 ,差异有统计学意义 (P <0 .0 1) ;P STAT1、P STAT6低表达 ,各组间差异无统计学意义。 (3)在血管内皮细胞中 ,VEGFR1的表达与P STAT5呈正相关 (r =0 .2 4 3) ,而VEGFR2与P STAT3呈正相关 (r =0 .30 8) ;在卵巢癌细胞中 ,VEGF、VEGFR1、VEGFR2与P STAT3、P STAT5之间均呈正相关 ,而与P STAT1、P STAT6无相关。结论VEGF可直接作用于卵巢上皮癌细胞 ,STATs可能参与了VEGF细胞内的信号传导。更多还原

【Abstract】 Objective To study the expressions of VEGF/VEGFRs and activation of STATs in ovarian epithelial carcinoma, and to elucidate direct effect of VEGF on ovarian carcinoma cells. Methods Tissue samples from 42 women with primary ovarian epithelial carcinoma (OVCA), 29 with begnin ovarian tumor (OVBT)and 11 with normal ovarian tissue (NOV)were collected. LSAB immunohistochemical staining was used to determine the expression of VEGF,VEGFR1,VEGFR2 and activated STATS (P STAT1、P STAT3、P STAT5、P STAT6) proteins. Results (1) Semi quantitative scoring showed that VEGF expression in OVCA was significantly higher than that in OVBT and NOV( P <0.01). Expressions of VEGFR1 and VEGFR2 were significantly elevated in OVCA, including tumor cells and stromal vascular endothelial cells( P <0.01, compared with OVBT and NOV). There was no difference in VEGFRs expressions between OVBT and NOV. (2) In OVCA, tumor cells and endothelial cells expressed P STAT3 and P STAT5 at significantly higher levels than those in OVBT and NOV ( P =0.000). The staining of P STAT1 and P STAT6 was weak with no significant differences among OVCA, OVBT and NOV. (3) Expressions of VEGFR1 and VEGFR2 in endothelial cells were significantly correlated with P STAT5 and P STAT3, respectively ( P =0.006 and 0.001). In cancer cells, VEGF, VEGFR1 and VEGFR2 were all CM(43 significantly orrelated with P STAT3 and P STAT5( P =0.000), but not with P STAT1 or P STAT6. CM) Conclusion VEGF affects ovarian carcinoma cells via VEGFRs, and STATs probably participate in intracellular signaling of VEGF.更多还原