【摘要】 目的　评价人乳头状瘤病毒脱氧核糖核酸(HPVDNA)第二代杂交捕获法(HC -Ⅱ)检测液基细胞学剩余标本中高危型(CINⅡ以上)HPVDNA用于筛查宫颈病变的可行性。方法　用HC Ⅱ方法分别对山西襄垣县研究组人群972份液基细胞检查剩余样品和对照组人群5 797份HPV直接取样标本进行高危型HPVDNA检测,以病理学为标准评定2次筛查结果。结果　2次筛查人群中HPV感染率分别为17 .5%和20 .1%。研究组HPVDNA检测检出宫颈高度病变以上的灵敏度和特异度为97 .2%和85. 6%;对照组为96 .6%和83 .2%。2种方法的差异无统计学意义(P>0 .05 )。结论　用HC II检测液基细胞学检查剩余标本中的HPVDNA,可替代HPV直接取样法,该方法也是一种有较好的子宫颈癌筛查方法。更多还原

【Abstract】 Objective To evaluate the feasibility of deoxyribonucleic acid testing with hybrid capture Ⅱ on the residue of liquid-based cytology to detect the patients with cervical intraepithelial neoplasia.Methods The data were from two population-based screening surveys in Xiang Yuan County. Hybrid capture Ⅱ was used to detect high risk HPV DNA on 972 specimens residue of thin-layer cytology as study group and 5 797 cervical specimens acquired by HPV samplers as control group. Pathological diagnosis was considered as gold standard to evaluate the two methods.Results The HPV prevalence result of two surveys were 17.5% and 20.1% respectively. Sensitivity and specificity were 97.2% and 85.6% for study group, 96.6% and 83.2% for control group.There was no difference between the rates.Conclusion HPV DNA test on the residue of liquid-based cytology can take the place of direct HPV test, which is a cost-effective screening method for cervical cancer.更多还原