【摘要】 目的　报告国内第一例由Arthrographiskalrae引起的真菌性全眼炎伴发侵袭性上颌窦、筛窦窦炎病例 ,并对眼内脓液中分离株进行鉴定。方法　从一例眼外伤患者左眼眶内清除组织及脓液中培养分离出真菌 ,并通过形态学、生化学包括尿素酶试验、放线菌酮耐受试验、最高生长温度测试、ID32生化鉴定板、药敏试验、组织病理学、辅酶Q分析和DNA序列分析等方法对该菌进行鉴定。结果　该菌形态学特征为具有双相型特点的菌落 ,镜下为典型链状关节孢子 ;生长温度最高可耐受4 2℃高温 ;对两性霉素B和伊曲康唑敏感。所含辅酶Q为辅酶Q 10 (H2 )。D1/D2和ITS区域的DNA序列分析 ,经正反链验证 ,分别确定了 6 0 4个和 5 0 8个碱基位点。通过日本国际核酸序列数据库 (DDBJ)进行同源性序列搜索证明 ,分离株与模式株UAMH36 16 (Arthrographiskalrae )的D1/D2和ITS区域有 10 0 %的碱基同源序列 ,证实为Arthrographiskalrae。 结论　这是国内首次鉴定Arthrographiskalrae,也是首次发现由该菌引起的真菌性全眼炎伴发侵袭性上颌窦、筛窦窦炎病例。更多还原

【Abstract】 Objective We isolated and identified a strain of Arthrographis kalrae from a patient with fungal panophthalmitis and invasive sinusitis involved maxillary and ethmoid sinus firstly in china. Methods The strain isolated from purulent material of injured left eye and identified based on gross and microscopic morphologies, biochemical assays, including urea hydrolysis test, the cycloheximide tolerance test, ID32 plate identification, ubiquinone system analysis, histopathological and susceptibility studies and DNA sequence analysis Results The isolate with a biomorphism characteristic could grow well at 25℃ and 37℃, and the tolerant growth temperature of this isolate was 42℃ Microbiologically, typical rectangular arthroconidia were mainly found The isolate was also confirmed to be susceptible to amphotericin B and itraconazole Ubiquinone system analysis revealed there ubiquinone 10 (H 2) Sequence on two LSUrDNA regions (D1/D2 and ITS) of the isolate and typical strain UAMH3616 (Arthrographis kalrae) were analyzed The sequences of regions, D1/D2 (604 bp) and ITS (508 bp) from the isolate and UAMH3616 were identical with 100% homology It was identified to be Arthrographis kalrae Conclusion This was the first identification of Arthrographis kalrae and first reported infection of panophthalmitis and invasive fungal maxillary and ethmoid sinusitis caused by Arthrographis kalrae in China更多还原