【摘要】 目的研究MHCⅠ类链相关基因A(MHCclassⅠchainrelatedgeneA,MICA)第5外显子微卫星多态性与湖南地区鼻咽癌之间的相关性。方法应用荧光聚合酶链反应基因扫描技术和聚合酶链反应序列特异性引物技术分析127例湖南地区鼻咽癌患者和112名正常人群MICA基因第5外显子微卫星等位基因及MICA基因缺失(MICADel)频率。结果MICAA9表型频率在患者组(45/127)高于对照组(20/112),相对风险值(relativerisk)为2.524(P=0.001,Pc=0.006);MICAA5.1表型频率在患者组(51/127)低于对照组(69/112),相对风险值为0.418(P=0.0004,Pc=0.0026)。进一步分析发现,MICAA9在男性患者组的表型频率(35/101)高于男性对照组(11/78),相对风险值为3.23(P=0.00095,Pc=0.006);MICAA5.1在男性患者组的表型频率(39/101)低于男性对照组(49/78),相对风险值为0.372(P=0.0007,Pc=0.004);各MICASTR等位基因频率在女性患者组与女性对照组之间的差异无统计学意义(Pc>0.05)。结论湖南地区MICASTR等位基因多态性与鼻咽癌相关,MICAA9是该人群男性个体的一个鼻咽癌遗传易感标记。更多还原

【Abstract】 Objective To explore the association between the short tandem repeat polymorphism of exon 5 of MICA gene(MICA-STR) and nasop hary ngeal carcinoma (NPC)in a southern Chinese population.Methods O ne hundred and twenty-seven consecutive NPC patients and 112 randomly selected normal controls residing in southern China mainland were analyzed for MICA-STR allelic variation and MICA gene deletion by fluorescent polymerase chain reacti on-genescanning and polymerase chain reaction-sequence specific priming. Results MICA*A9 was observed at significantly higher frequency in th e NPC patient group than in the control group (relative risk=2.524,P=0.001, Pc=0.006); whereas MICA*A5.1 was present at significantly lower frequency in the NPC patient group than in the control group(RR=0.418,P=0.0004, P c=0.002 6). Further analysis revealed that MICA*A9 was over-represented in male NPC pat i ents, compared with male controls(RR=3.23,P=0.00095,Pc=0.006); wher eas MICA*A5.1 was present at significantly lower frequency in male NPC patients, compared with male controls(RR=0.372,P=0.0007,Pc=0.004).None of t he MICA-STR variants showed statistically significant frequency difference bet ween female NPC patients and female controls(Pc>0.05).Conclusion MICA-STR polymorphism is associated with NPC, and MICA*A9 is a genetic susce p tibility marker of male individuals for NPC in a southern Chinese population.更多还原