【摘要】 目的制作睾丸组织异体异位移植动物模型,并观察原始生精细胞在异体异位继续生长、发育情况。方法将鼠龄为1～2d昆明小鼠睾丸组织移植至去势裸鼠背部(n=29),于移植后30～110d取出移植物,观察移植物的生长情况、表观形状以及各级生精细胞、生精上皮发育的期相情况。结果术后29只受鼠全部存活,在其背部均观察到移植物的生长,移植物直径由移植前的(0.73±0.05)mm增加到(6.75±0.73)mm,湿重由移植前(5.67±0.72)mg增加到(113.12±78.23)mg,受鼠皮肤血管已广泛深入到移植物内;光镜下可见移植物中生精小管结构清晰,移植物与受鼠皮肤之间建立了紧密连接,移植物中含有各级生精细胞及精子,在过碘酸Schiff(PAS)染色的移植物标本可见整个精子发生周期(从StageⅠ到StageⅫ)各阶段的生精细胞。结论新生小鼠睾丸组织移植到去势裸鼠背部能够继续生长、发育,可作为研究生精细胞增殖发育规律和精子发生调控机制的动物模型。更多还原

【Abstract】 Objective To observe the growth and the development of primordial spermatogenic cells by allografting Kun-ming neonatal mouse testes into athymic nude mice.Methods Neonatal mouse testes were grafted under the back skin of nude mice (n=29). Grafts were taken out at the post-grafting day 30-110 and observed on the growth and the appearance, the structure of seminiferous tubules as well as the development of spermatogenic cells and the stage of seminiferous epithelium.(Results) The graft growth in all 29 recipient animals was observed, and the mass of grafts was significantly increased from (0.73)±(0.05) mm in diameter and (5.67)±(0.72) mg in wet weight pre-grafting to (6.75)±(0.73) mm and (113.12)±(78.23) mg post-grafting, respectively. The structure of seminiferous (tubules) inside grafts was clearly seen under a light microscope. And the histological analysis (demons)-(trated) a complete spermatogenesis with all levels of spermatogenic cells including sperm and stage I-XII of the seminiferous epithelium could also be identified.Conclusion Neonatal mouse testes could continuously grow and develop under the back skin of castrated nude mice, which would therefore be a useful tool for investigations on the proliferative and developmental pattern of spermatogenic cells and the regulation mechanism of spermatogenesis.更多还原