【摘要】 目的:观察川芎内酯A预处理对大鼠离体心脏缺血再灌注所致血管内皮细胞损伤的保护作用。方法:采用Langendorff离体心脏灌流方法,在恒温恒压条件下,以0.5mL.min-1的灌流速度给药,剂量分别为0.05,0.025,0.0125g.L-1与K-H灌流液同时灌流心脏10min;全心停灌30min,再灌60min。结果:川芎内酯A预处理能提高大鼠离体心脏停灌再灌期的冠脉流量;降低冠脉流出液中IL-1β浓度和TXB2/6-Keto-PGF1α的比值;室性心动过速(VT)和心室颤动(VF)的发生率降低,VT,VF持续时间缩短;心肌匀浆液乳酸脱氢酶、丙二醛的含量降低,超氧化物歧化酶活性提高。结论:川芎内酯A预处理对大鼠离体心脏停灌再灌注损伤所致的血管内皮细胞损伤可能具有保护作用。更多还原

【Abstract】 Objective: The aim of this study was to investigate the protective effect of Chuanxiong-pathalide A on the injury of endothelial cell induced by ischemia and reperfusion in isolated rat hearts. Method: The isolated rat hearts were perfused under constant pressure with Chuanxiong-pathalide A at the concentrations of 0.012 5 mg·mL^-1, 0.025 mg·mL^-1 and 0.05 mg·mL^-1 within 10 min followed by a 10-min washout period before the induction of a 30-min normothermic global ischemia and 60 min reperfusion. Result: Pretreatment with Chuanxiong-pathalide A produced a reduction in the incidence of reperfusion-induced ventricular fibrillation （VF） and ventricular tachycardia （VT）. Pretreatment of the hearts with high dose of Chuanxiong-pathalide A (0.05 mg·mL^-1) prior to the ischemia and reperfusion, reduced the incidence of reperfusion-induced VF and VT to 37.5% as compared with non-pretreated control group （P<0.05）. The duration of occurrence of VF and VT in the group pretreated with Chuanxiong-pathalide A at dosages of 0.012 5 mg·mL^-1, 0.025 mg·mL^-1 and 0.05 mg·mL^-1 were （7.50±1.61）, （1.64±0.67） and （1.06±0.70） min respectively, which were significantly shorter than （23.51±3.99） min in non-pretreated control group; 0.05 mg·mL^-1 of Chuanxiong-pathalide A increased coronary flow in comparison with the control group. The content of SOD in the group pretreated with 0.05 mg·mL^-1 of Chuanxiong-pathalide A was （59.6±18.7） U·mg^-1, significant lower than （92.3±19.0） U·mg^-1 in the non-pretreated group. The contents of LDH and MDA were （2 378.0±196.3） U·g^-1 and （12.1±1.3） nmol·mg^-1 in the non-pretreated group, which were much higher than the values of （1 669.4±192.5） U·g^-1 and （6.9±0.8） nmol·mg^-1 in the group pretreated with Chuanxiong-pathalide A respectively. In addition, enzymeimmunoassays showed an decreased production of IL-1β and the ratio of TXB₂/6-Keto-PGF_1α. Conclusion: Our results show that chuanxiong-pathalide A pretreatment can protect the endothelial function from the injury caused by ischemia and reperfusion.更多还原