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HPLC法测定蓝芩口服液中的栀子苷含量
Determination of Gardenoside in Lanqin Oral Liquid by HPLC
【摘要】 建立高效液相色谱法测定蓝芩口服液中的栀子苷含量的方法。色谱柱:Kromasil C18柱;流动相:0.05mol.L-1磷酸氢二钠-甲醇(70:30);流速1.0ml.min-1;检测波长238nm。栀子苷在9.6~48.0μg.ml-1之间峰面积与浓度呈良好的线性关系,r=0.9999,平均回收率为98.5%(n=6),RSD为0.66%。该方法可用于蓝芩口服液中栀子苷的质量控制。
【Abstract】 A HPLC method for the determination of geniposide in Lanqin Oral Liquid was established.A Kromasil C18 column was used with 0.05mol·L-1 Na2HPO4 and methanol(70∶30) as the mobile phase.The flow rate was 1.0ml·min-1,!and the detecting wavelength was 238nm.There was a good linear relationship(r=0.9999) when the concentration range of geniposide was 9.6~48.0μg·ml-1.The average recovery was 98.5%(n=6),RSD=0.66%.The method could be used in quality control of geniposide in Lanqin Oral Liquid.
- 【文献出处】 中国药事 ,Chinese Pharmaceutical Affairs , 编辑部邮箱 ,2005年11期
- 【分类号】R286.0;
- 【被引频次】7
- 【下载频次】161