【摘要】 根据已发表的鹅副黏病毒基因组序列,设计并合成了扩增F基因和HN基因的5对引物,利用RT-PCR的特异性扩增出了广东省清远分离株(QY株)的F基因和HN基因。然后将其克隆入pMD 18-T载体,经鉴定、测序及拼接,QY株的F基因和HN基因全序列长度分别为1 662bp和1 716 bp,分别编码553个和571个氨基酸。经与GenBank登录的几株参考毒株F基因和HN基因编码区的核苷酸序列进行比较;结果,QY株与参考毒株SF02株和LaSota株F基因的核苷酸序列同源性分别为98.3%和82.4%,HN基因的核苷酸序列同源性分别为97.2%和75.9%。更多还原

【Abstract】 According to the published genome sequence of goose’s paramyxovirus,five pairs of primers were designed to amplify F gene and HN gene from goose’s paramyxovirus Qingyuan(QY) strain(from Guangdong Province) by RT-PCR.The PCR products were cloned into pMD 18-T vector,identified and sequenced.Nucleotide sequence of F and HN genes of the QY strain was 1662bp and 1716bp in length respectively,and had a single open reading frame,encoding 553 and 571 amino acids respectively.In comparison with the published strains SF02 and LaSota from GenBank,the nucleotide homology of F gene were 98.3% and 82.4% respectively,and the nucleotide homology of HN gene were 97.2% and 75.9% respectively.更多还原