【摘要】 目的探讨光动力学疗法(photodynamictherapy,PDT)与白花蛇舌草联合应用对大鼠C6胶质瘤细胞的作用。方法常规培养的Wistar大鼠C6胶质瘤细胞用MTT法、吖啶橙/溴乙啶(AO/EB)双荧光染色法和流式细胞术观察PDT联合白花蛇舌草对体外培养的大鼠C6胶质瘤细胞存活率的影响、检测凋亡、分析对细胞形态学及细胞周期的影响。结果白花蛇舌草可明显抑制C6胶质瘤细胞的生长;使肿瘤细胞聚集在S期并诱导其凋亡。PDT与各浓度白花蛇舌草联合可抑制C6胶质瘤细胞增殖(P<0.01)。白花蛇舌草和PDT均可在一定程度上诱导C6胶质瘤细胞凋亡,二者联用后诱导细胞凋亡作用显著增强,这种作用在白花蛇舌草浓度为5.0μg/ml作用12h时最明显。结论白花蛇舌草可与PDT协同抑制C6胶质瘤细胞的增殖。其协同作用的机制可能与诱导细胞凋亡有关。更多还原

【Abstract】 Objective To study the influence of PDT combined with Hedyotis diffusa in vitro on the anti-proliferative effect of Wistar rat glioma cell line C6. Methods MTT assay, acridine orange （AO）/ethidium bromide（ED） fluorescent staining and flow cytometry（FCM） were used to investigate the influence on C6 cells growth, observe apoptosis, analyze cell cycle and ultrastructure changes after treated by PDT and Hedyotis diffusa in vitro. Results Hedyotis diffusa was effective in inhibiting the proliferation C6 cells and could block cells in G₀/G₁ phases and induce cell apoptosis. These effects could be enhanced significantly by PDT（P<0.01）. The Hedyotis diffusa and PDT could all induce cell apoptosis.When they combined, the inductive effect for cell apoptosis became more obvious at 5.0μg/ml of Hedyotis diffusa after 12 hours. Conclusions PDT can enhance the antiproliferative effect of Wistar rat glioma cell line C6. The synergestic antiproliferative effect of Hedyotis diffusa and PDT may be the result of cell apoptosis induction.更多还原