【摘要】 目的利用16 s-23 s rRNA间隔区(ITS)的多态性,对中国布鲁氏菌种间或种内生物型进行鉴别,评价ITS作为基因标识物的意义,寻找适合布鲁氏菌分型研究的基因标识物。方法应用聚合酶链反应-单链构象多态性(PCR-SSCP)分析技术,对中国120株布鲁氏菌的ITS进行分析和筛选,测序结果与Genbank中的布鲁氏菌ITS序列进行比较分析。结果对16 s-23 s rRNA间隔区的SSCP结果分析,得到4种不同的带型(ⅠI、I、Ⅲ、Ⅳ),测序序列有3个位点的差异,达到99.87%的一致性。结论中国布鲁氏菌的ITS序列高度保守,具有一定探讨其作为布鲁氏菌属种内分型的基因标识的价值。更多还原

【Abstract】 Objective To identify Brucella spp by 16s-23s rRNA Ribosomal-Spacer-Region(ITS) and to estimate the significance of ITS as gene labelling in Brucella.Methods Strain typing of 120 Brucella spp.in China by PCR-SSCP analysis and sequencing for ITS.Results Four types(I,Ⅱ,Ⅲ,Ⅳ) existed in all species which were examined.Sequence analysis of the ITS revealed a spacer of 765 bp with high(99.87%) homology and only 3 sites were different.Conclusion ITS with high homology is of some importance to be marker in Brucella spp.更多还原