【摘要】 目的探讨碘过量对体内、体外甲状腺钠碘转运体(NIS)mRNA表达的影响。方法Wistar大鼠,随机分为低碘组(LI)、适碘组(NI)、5倍高碘组(5HI)、10倍高碘组(10HI)、50倍高碘组(50HI)、100倍高碘组(100HI),检测尿碘、甲状腺NISmRNA表达水平。FRTL细胞分别在含有10-6～10-3mol/L碘化钾的培养基中培养24、48h,检测NISmRNA水平的变化。结果LI组尿碘显著低于NI组,而甲状腺NISmRNA表达水平明显高于NI组(P<0.01);各高碘组尿碘与NI组比较呈成倍升高,NISmRNA水平与NI组相比逐渐下降。FRTL细胞在分别含有10-6～10-3mol/L碘化钾的培养基中培养24、48h,NISmRNA的表达水平与对照组差异无统计学意义(P>0.05)。结论碘对体内、体外甲状腺NISmRNA表达的影响存在不同的机制,长期、慢性处于高碘摄入的大鼠主要通过转录水平影响甲状腺NISmRNA的表达,而体外急性实验表明,高碘则可能通过转录后水平而起作用。更多还原

【Abstract】 Objective To explore the effects of iodide on sodium iodide symporter(NIS) mRNA expression in vivo and in vitro. Methods Wistar rats were divided into low iodine group(LI),normal iodine group(NI),five-fold high iodine group(5HI),ten-fold high iodine group(10HI),fifty-fold high iodine group(50HI) and one hundred-fold high iodine group(100HI). After the rats were fed three months, urinary iodine and thyroid NIS mRNA expression were measured. FRTL cells were incubated in various concentrations of potassium iodide(10-6 ～ 10-3 mol/L). After 24 or 48 hour,NIS mRNA was measured. Results Urinary iodine was significantly lower in LI than in NI,whereas NIS mRNA expression was obviously elevated in LI than in NI(P < 0.01);The urinary iodine in 5HI,10HI,50HI and 100HI increased in folds compared with NI. The expressions of NIS mRNA also showed a decreasing tendency compared with NI. Iodide had no effect on the expression of NIS mRNA in FRTL cells on the 24th and 48th hour compared with cells cultured without iodide. Conclusions There are different mechanisms for iodide regulating thyroid NIS mRNA expression in vivo and in vitro, either at transcriptional level in rats with chronic long-term iodine excess intake,or possibly at post-transcriptional level in FRTL cells with acute iodine excess.更多还原