【摘要】 目的:采用非水反相液相色谱法测定大鼠血浆中大黄素、大黄素甲醚的含量。方法:血浆经水解、提取后用非水反相高效液相色谱法分析其含量,采用KromasilC18色谱柱 (250mm×4. 6mm, 5. 0μm),甲醇 冰醋酸 ( 99. 9∶0. 1 )为流动相;流速为 1. 0mL·min-1;检测波长 254nm。结果:大黄素在 0. 0425~2. 8μg·mL-1、大黄素甲醚在 0. 0491~3. 14μg·mL-1范围内线性关系良好;大黄素、大黄素甲醚的回收率分别为 95. 7 ~100. 1 , 96. 2 ~99. 8 ,RSD分别为1. 3 , 1. 6 ;提取回收率分别为 91. 8 ~95. 4 、92. 7 ~94. 6 。结论:本方法准确、简便,重现性好,适宜于测定含大黄素、大黄素甲醚的生物样品。更多还原

【Abstract】 AIM: To determine the content of emodin and physcion in rat plasma by nonaqueous RP-HPLC. METHODS: After hydrolysis and extraction, the content of emodin in the plasma was determined by nonaqueous RP-HPLC. The separation was performed on Kromasil C 18 column (250 mm×4.6 mm, 5.0 μm) with the mobile phase comprised of methanol-acetic acid (99.9∶0.1). The flow rate was 1.0 mL·min -1 and the detection wavelength was at 254 nm. RESULTS: The linear ranges for emodin and physcion were in the range of 0.0425-2.8 μg·mL -1 and 0.0491-3.14 μg·mL -1 , respectively. The average recoveries of emodin and physcion were 95.7%-100.1% and 96.2%-99.8%, with corresponding RSD of 1.3% and 1.6% respectively. CONCLUSION: This method is simple, rapid, accurate and reproducible with RP-HPLC to detect rhein in plasma.更多还原