【摘要】 目的 :研究雌二醇 (17β -estradiol,E2 )对人外周血来源树突状细胞 (DCs)成熟和免疫学功能的影响。方法 :在人外周血来源DC体外培养时加入两种浓度的E2 (10 -7mol/L和 10 -6mol/L)处理 ,光镜和电镜下观察DCs的生成情况及形态变化 ,以流式细胞仪分析各组细胞的免疫表型 ,用ELISA方法测定其分泌的IL - 12水平 ,[3 H]-TdR掺入法检测体外混合淋巴细胞反应中DCs刺激同种反应性T细胞的增殖能力。结果 :加入E2 培养后 ,DCs树突状和膜面状伪足减少 ,表达低水平MHC -II分子和共刺激分子CD4 0、CD80和CD86 ,其分泌的IL - 12水平明显下降 ,DCs刺激同种反应性T细胞的功能显著下降。结论 :E2 抑制人外周血来源DC的成熟 ,对其免疫学功能具有负性调节作用。更多还原

【Abstract】 AIM: To investigate the effects of 17β-estradiol (E 2) on the maturation and immunologic function of dendritic cells from human peripheral blood. METHODS: Cultured DCs were treated with E 2 at doses of 10 -7 mol/L and 10 -6 mol/L. The morphologic changes were observed under the scanning electronic microscope. The immunophenotype of DCs in control and treated groups was analyzed by flowcytometry. IL-12 production in culture supernatant was examined by ELISA assay. The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by incorporation of 3H]-TdR. RESULTS: Compared with control group, DCs cultured in the presence of E 2 displayed less dendritic pseudopod, expressed low levels of MHC-II, CD40, CD80 and CD86, and exhibited weakly activity in stimulating the proliferation of allogeneic T cells and reduction of IL-12 production. CONCLUSION: E 2 exerts a negative effect on the maturation and immunologic function of dendritic cells from human peripheral blood.更多还原