【摘要】 目的探讨兔髂动脉急性损伤后,氟伐他汀对血管内膜增殖的抑制作用及机制。方法给新西兰大耳白兔行右髂动脉球囊导管内膜剥脱术,术后灌胃予氟伐他汀(8mg·kg-1·d-1)治疗28d,术前、术后3h及3d用放射免疫法测定血清中内皮素-1、血栓素A2和前列环素浓度;28d后HE染色观察血管内膜增生情况,免疫组织化学方法检测细胞外基质的变化。结果①兔髂动脉急性损伤后血清内皮素-1及血栓素A2浓度即明显增加,前列环素水平则显著下降;氟伐他汀治疗显著抑制内皮素-1和血栓素A2的升高;提高血清前列环素的水平;②氟伐他汀治疗减轻了内膜TGF-β1的过度表达及细胞外基质(FN、LN)的沉积;③氟伐他汀显著抑制了内膜的增殖和肥厚。结论氟伐他汀治疗抑制血管损伤后血栓的形成,减轻了内膜的增殖及内膜中细胞外基质的沉积。更多还原

【Abstract】 AIM: To investigate the effects of fluvastatin on intimal hyperplasia following balloon injury of rabbit iliac artery. METHODS: The iliac arteries of twelve white New Zealand rabbits were injured with an inflated balloon catheter, six rabbits were given fluvastatin 8 mg·kg^-1 ·d^-1 by gavage, the others served as controls. Serum concentrations of endothelin, thromboxane A₂ and prostacyclin were measured by radioimmunoassay at time points before, 3 hours and 3 days after balloon injury. After 28 days, ratio of intima-to-media area （I/M） of the injured arteries were calculated using a computer image analysis system and the alteration of TGF-β₁ and extracellular matrix （FN, LN） were quantified with immunohistochemistry. RESULTS: ① Serum endothelin-1 and thromboxane A₂ significantly increased after injury, whereas serum prostacyclin markedly decreased. The alterations were completely reversed by the treatment with fluvastatin. ② The expression of TGF-β₁ and deposition of ECM in intimal were much lower than that in the untreatment animals. ③ Intimal hyperplasia after balloon injury was significantly attenuated by fluvastatin treatment. CONCLUSION: The inhibitory effects of fluvastatin on intimal hyperplasia after balloon injury may attribute to its inhibiting thrombosis and deposition of ECM. [更多还原