【摘要】 从南京某石化厂排污口附近采集土样,以菲为碳源的选择性培养基分离筛选到一株菲高效降解菌F10a,根据形态和生理生化特性初步鉴定为芽孢杆菌属,并对其降解菲的特性及各种影响因素进行了研究.结果表明,F10a在50mg.L-1的条件下,28℃振荡培养27h,菲的降解率达到98.12%;静置培养84h,菲的降解率达到98.47%.pH值分别为4、6、8时,F10a对菲具有良好的降解效能;pH值为10时F10a不生长.Zn2+与Pb2+的存在不影响F10a的降解效能,Cu2+可以延缓菲的降解,Cr2+对F10a有毒性.F10a在菲浓度为200mg.L-1时,28℃振荡培养84h,降解率为99.6%.菲的降解程度与细菌数量的增长呈正相关关系.更多还原

【Abstract】 Several PAHs-degrading bacteria were isolated from the soil near a petrochemicals factory,and one strain F10a identified as B.sphaericus was chosen for use.The study on the phenanthrene-degradation potential of the strain and its affecting factors showed that at 28 ℃,the degradation rate of phenanthrene (50 mg·L -1 ) was 98.12% after 27 hours rotary culture,and 98.47% after 84 hours static culture.F10a had a good phenanthrene-degradation capability when the pH was 4,6 and 8,but its growth was inhibited when pH was 10.Cr 2+ was toxic to the strain,Cu 2+ could delay the degradation of phenanthrene,while Zn 2+ and Pb 2+ had no significant effects.The degradation rate of phenanthrene (200 mg·L -1 ) was 99.6% after 84 hours rotary culture.A significant positive relationship was found between bacterial growth and phenanthrene degradation.更多还原