【摘要】 目的　通过利用对甲肝病毒(HAV)RT -PCR产物的半定量分析建立快速检测甲肝减毒活疫苗滴度的方法,并比较该方法与细胞培养(CCID50 )检测法的相关性。方法　运用RT -PCR的方法,以保守的HAVVP3-VP1部分基因区为目标区对甲肝疫苗病毒进行核酸扩增,利用PharmaciaImageMaster○RVDS凝胶成像分析系统进行产物的半定量分析得出结果,并与常规细胞培养法所测感染性滴度(CCID50 )进行比较。结果　本检测方法比细胞培养法缩短了2 0余天,与细胞培养法的灵敏度相似,与细胞培养法结果的差异无统计学意义。结论　所建立的方法具有操作简便、快速的优点,有望替代细胞培养法作为用于检测甲肝减毒活疫苗滴度的常规方法。更多还原

【Abstract】 Objective A swift method for evaluating the titer of HAV live attenuated vaccine was developed by semi-quantifying analyzing the RT-PCR product of HAV.The relationship between this method and cell culture method〔cell culture infective dose 50 percent (CCID_ 50 )〕was also analyzed.Method Using reverse transcription and polymerase chain reaction techniques and the half-quantitative function of Pharmacia ImageMasterD○R VDS and analying software,the titer of HAV live attenuated vaccine was tested.The highly conserved encoding part region of the capsid protein (from VP3 to VP1)was selected for amplification.The results were compared with those obtained by CCID_ 50 .Result The sensitivity and specificity between the above method and cell culture method were conformable.Conclusion The above method is more swift and convenient,and has the potential to be used to measure the titer of HAV live attenuated vaccine instead of traditional method.更多还原