【摘要】 目的观察苯那普利对实验性糖尿病大鼠肾小管-间质血小板源性生长因子-B(PDGF-B)表达的影响。方法大鼠随机分为单侧肾切除组、单侧肾切除糖尿病组及苯那普利给药组。4周后应用荧光分光光度法测定血浆与肾组织ACE活性,点计数技术观察肾小管-间质形态学变化,免疫组化方法检测肾小管-间质Ⅳ型胶原及PDGF-B蛋白表达。结果苯那普利给药4周对糖尿病大鼠血浆及肾组织ACE活性抑制分别达92.00%与88.77%,并可缓解糖尿病大鼠肾小管-间质异常损害;免疫组化证实其对肾小管基底膜Ⅳ型胶原及肾小管-间质PDGF-B蛋白表达有明显抑制作用。结论苯那普利对糖尿病大鼠肾小管-间质损害有明显抑制作用,部分机制是通过抑制肾小管-间质PDGF-B蛋白表达。更多还原

【Abstract】 Objective To investigate the effect of benazepril on expression of PDGF-B in the renal tubulointerstitium in experimental diabetes. Methods Rats were randomly divided into control, diabetic, and diabetic treated with benazepril 〔10 mg/(kg·d), by gavage〕 groups. 4 weeks after treatment, ACE activity in plasma and renal tissue was measured by fluorimetric assay. Tubulointerstitial morphological analysis was performed in HE stained sections by a point technique. The expressions of type Ⅳ collagen and PDGF-B protein were determined by immunohistochemistry. Results ACE activities in plasma and renal tissue were reduced by benazepril by 92.00% and 88.77% respectively. The proportion of normal tubules was substantially reduced in the diabetic group as a result of tubular dilatation. Benazepril resulted in amelioration in tubulointerstitial damage. Greater immunostaining for type Ⅳ collagen was present in the tubular basement membrane of diabetic rats compared with that of benazepril treated diabetic rats (P<0.05). PDGF-B immunostaining was found in greatest abundance in renal tubulointerstitium within epithelial cells of the proximal tubule and distal tubule; while in benazepril treated diabetic rats only sparse PDGF-B was found in the tubular epithelium (P<0.05). Conclusion The protective effects of benazepril on renal tubulointerstitium are associated, at least partly, with down-regulating overexpression of PDGF-B.更多还原