【摘要】 目的探讨血管紧张素Ⅱ(AngⅡ)对人α1(Ⅰ)胶原基因启动子活性的调控作用和川芎嗪干预的影响环节。方法将2.5kb长度的人α1(I)胶原基因启动子片段与氯霉素乙酰基转移酶(CAT)“报告基因”组成的重组体pCOLH2.5,采用脂质体法转染至培养的大鼠心肌成纤维细胞,分别加入AngⅡ和川芎嗪,ELISA法测定CAT活性。结果AngⅡ可明显促进pCOLH2.5片段的启动活性,川芎嗪能抑制AngⅡ的促pCOLH2.5启动活性(P<0.05)。结论川芎嗪能在转录水平上阻断AngⅡ诱导的人α1(I)胶原基因启动子活性增加,从而抑制I型胶原的合成,阻滞和延缓心肌纤维化的发生发展。更多还原

【Abstract】 Objective To study the roles of tetramethylpyrazine on regulation of collagen gene promoter induced by angiotensin Ⅱ. Methods Cardiac fibroblasts of rat transfected by pCOLH 2.5, which were composed of 2.5 kb length of α1 type I collagen gene promoter segments and chloramphenicol acetyltransferase (CAT), were added angiotensin Ⅱ and tetramethylpyrazine. The activity of CAT was measured by ELISA technique. Results AngⅡenhanced the activity of pCOLH2.5, tetramethylpyrazine could inhibited the transcriptional activity of the promoter increased by AngⅡ(P <0.01). Conclusion Tetramethylpyrazine could decrease the promoter activity of the humanα1 (Ⅰ) gene, and inhibit the composition of collagen I, against the myocardial fibrosis.更多还原