【摘要】 选用重庆市大面积推广的优良甘薯品种进行茎尖脱毒离体培养试验.结果表明:对来源于薯块萌发芽的茎尖进行组织培养,其成苗率最高,达53.2%;连续两次进行茎尖培养可明显增强脱毒效果;对于甘薯病毒病的检测,反转录PCR法(RT-PCR)比硝化纤维素膜酶联免疫吸附检测法(NCM-ELISA)更灵敏和可靠.更多还原

【Abstract】 In this paper, shoot-tip culture in vitro for eliminating virus was studied by using the excellent sweet potato cultivars which widely spread in Chongqing as the test materials. The results showed that the frequency of plant regeneration of the stem apex which sprouted directly from tuber was the highest, and reached 53. 2%; continuous shoot-tip culture twice can apparently improve the effects of virus elimination; the method of reverse transcription PCR (RT-PCR) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA).更多还原