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小麦雌性不育基因的SSR多态性标记
Research of the Sterile Gene for Female Sterile Wheat by Microsatellite Marker
【摘要】 以普通小麦新601×雌性不育小麦XND126的F2群体作为育性调查以及基因标记群体。通过对育性基因的分析,确定在此组合中雌性不育基因表现为1对主效基因控制;结合混合分组分析法(Bulk Segregant Analy-sis,BSA),首次对小麦雌性不育基因进行了SSR标记研究,通过对一千对微卫星引物的筛选,初步确定了微卫星引物cfd36和gwm296与主效基因连锁。
【Abstract】 A study was carried out through the genetic analysis of F2 population which is derived from a common wheat(new 601)and the female sterile line(XND126).The result indicated that the sterile gene was performed by a pair recessive gene in the cross.The F2 population was used to mark the female sterile gene by microsatellite and BSA(bulked segregant analysis) at the first time.Among the 1,000 pairs of microsatellite primers,two markers were found to be polymorphic between the two pools and the two parents.The experimental research showed that microsatellite marker cfd36 and gwm296 were linked with the major sterile gene.
- 【文献出处】 新疆农业大学学报 ,Journal of Xinjiang Agricultural University , 编辑部邮箱 ,2005年03期
- 【分类号】S512.1
- 【被引频次】9
- 【下载频次】204