【摘要】 采用生化方法对锯缘青蟹(Scylla serrata)凝集素进行了初步研究.结果表明,在锯缘青蟹体内具有血球凝集活性的凝集素.凝集素不仅分布于肌肉组织,同时分布于血清中,且两者在热稳定性、pH适应性、对金属离子的依赖性等方面都存在着差异.分别向肌肉提取液和血清加入50%的固体硫酸铵使凝集素沉淀,用不同饱和度的硫酸铵溶液依次对两沉淀物进行抽提,抽提物的比活力分别提高了约2.25倍和1.27倍,总活力回收分别为33.36%和16.70%.更多还原

【Abstract】 Lectin of Scylla serrata was studied by biochemistry methods.The results showed that lectins distributed not only in the muscle but also in the serum of Scylla serrata.There were lots of different properties between these two lectins, such as thermostability,the range of pH and the dependence on metal ionic.Muscular extract agglutination quail erythrocytes depended on Ca²⁺ evidently,and it keep 100% agglutinative activity in the range of pH 5.0～8.0,it had agglutinative activity in temperature range of 4～90℃.Agglutinative activity of serum to quail erythrocytes also depended on Ca²⁺,but it still had agglutinative activity when EDTA had added up to 50 mmol/L,it revealed that the degree of depending on Ca²⁺ is small compare with muscular extract.And it had higher agglutinative activity in the range of pH 7.0～9.0,its agglutinative activity lost at 80℃ by 10 minutes.Adding solid ammonium sulfate into the muscles and serums up to 50% saturation at 4 ℃,the precipitates were continually extracted by ammonium sulfate with different concentration.The extractions from muscle and serum given a 2.25-fold and 1.27-fold purification individually,（33.36%） and 16.70% recovery of total activities compared with the muscle and serum.更多还原