【摘要】 目的:通过测定子痫前期-子痫患者胎盘组织中内皮型一氧化氮合酶运输介导物(endothelial nitric oxide synthase traffic inducer,NOSTR IN)和内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)的表达,探讨它在子痫前期-子痫发病中的作用。方法:采用逆转录-聚合酶链反应(RT-PCR)检测21例子痫前期-子痫患者(病例组)和17例正常晚孕妇女(正常组)胎盘组织中NOSTR IN mRNA的表达;W estern blot检测胎盘组织中NOSTR IN和eNOS蛋白质的表达;分光光度法测定胎盘组织中eNOS的活性;应用硝酸还原酶法测定孕妇静脉血中的NO代谢产物亚硝酸基/亚硝基(NO2-/NO3-)。结果:病例组患者胎盘组织中NOSTR IN mRNA呈强表达,明显高于正常组(P<0.01);W esternblot显示两组胎盘组织中均有58kDa的NOSTR IN蛋白质表达,但病例组表达量明显增高(P<0.01),同时也均有145kDa的eNOS蛋白质表达,两组表达量比较无差异(P>0.05);病例组患者胎盘组织eNOS活性为13.727±3.58 U/mg,与正常组的21.69±3.84U/mg比较降低显著(P<0.01);病例组孕妇外周血清NO2-/NO3-为38.56±8.49μmol/l,与正常组的65.37±9.61μmol/l比较显著降低(P<0.01);病例组胎盘组织中NOSTRN表达水平与eNOS活性呈负相关(r=-0.57,P<0.01)。结论:子痫前期-子痫患者胎盘组织中NOSTR IN表达水平升高,eNOS活性降低,这可能在子痫前期-子痫的发病中起重要作用。更多还原

【Abstract】 Objective:To investigate the expression of endothelial nitric oxide synthase traffic inducer（NOSTRIN） and endothelial nitric oxide synthase（eNOS） in the placentas of the patients with pre-eclampsia and its role in the pathogenesis of pre-eclampsia.Methods:Reverse transcript polymerase chain reaction（RT-PCR） was used to detect the expression of NOSTRIN-mRNA in placental tissue of 21 pre-eclampsia patients（pre-eclampsia group） and 17 normal pregnant women（normal group）;Western blot analysis was applied to detect NOSTRIN and eNOS expression.The eNOS activity in placental tissues was assayed by spectrophotometry.Serum（NO₂）^-/（NO₃）^-,the stable metabolic end product NO,was measured using nitrate reductase.Results:The expressive quantity of NOSTIN mRNA was significantly higher in women with pre-eclampsia than in the normal group（P<0.01）;Western blot analysis showed that both groups express 58kD NOSTRIN but the protein level of NOSTRIN was significantly higher in women with pre-eclampsia than in the normal group（P<0.01）.However,no significant difference between the two groups was observed in the expression of placental eNOS（P>0.05）;The activity of eNOS was significantly decreased in pr-eclampsia group（13.727±3.58 U/mg） compared with the normal group（21.69±3.84 U/mg）（P<0.01）;The levels of serum（NO₂）^-/（NO₃）^-were significantly lower in women with pre-eclampsia（48.56±8.49μmol/l） than in the normal group（65.37±9.61μmol/l）（P<0.01）.A significant negative correlation existed between the expression of NOSTRIN protein and the activity of eNOS in placental tissues of women with pre-eclampsia（r=-0.57,P<0.01）.Conclusion:The level of NOSTRIN expression in the placental tissues of women with pre-eclampsia is increased and is negatively correlated with the activity of eNOS,indicating that this may play an important role in the pathogenesis pre-eclampsia.更多还原