【摘要】 水稻芽期冷害是我国长江中下游的早稻种植区和东北、西北稻区及云贵高原的一季稻区水稻生产中的重要限制因子之一。研究中利用纸卷法测定1个水稻重组自交系群体对10℃低温的芽期耐冷性,结合1张高密度分子遗传图谱,进行QTL定位分析。检测到控制水稻芽期耐冷性的4个QTL,分别位于1、3、7和11号染色体上。其中,位于11号染色体上的QTLqSCT-11的效应最大,在10℃低温处理13d时,对性状的贡献率达26%～30%,被检测到的LOD值也高达16～19,其加性效应值为正,增效等位基因存在于亲本Lemont中,RM202为与QTLqSCT-11紧密连锁的SSR标记。该主效QTL的增效基因,可作为分子标记辅助选择的操作对象用于水稻芽期耐冷性的遗传改良。更多还原

【Abstract】 Cold stress is one of the major restraints for stable stand establishment at the early seedling stage of rice (Oryza sativa L.) for much of the production area. A recombinant inbred line population derived from a japonica/indica cross were assessed for cold tolerance at early seedling stage by the paper-roll tests at 10°C. Main-effect QTL for the trait were mapped via composite interval mapping using a linkage map with 198 marker loci. Four putative QTL for the trait were identified, which were mapped to chromosomes 1, 3, 7 and 11, respectively. Among these QTL, qSCT-11 showed a large additive effect on the trait, explaining 26%-30% of the phenotypic variation in the treatment of 13 d at 10℃ with a LOD of 16-19. This QTL was closely linked to SSR marker RM202 and its positive allele came from the parent Lemont. This allele would be useful for the improvement of rice cold tolerance via marker-assisted selection.更多还原